• Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g POLR1E antibody (STJ114573). Western blot was performed from the immunoprecipitate using POLR1E antibody (STJ114573) at a dilution of 1:1000.
  • Western blot analysis of various lysates using POLR1E Rabbit polyclonal antibody (STJ114573) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POLR1E antibody (1-419) (STJ114573)

SKU:
STJ114573

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLR1E (1-419) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR1E
Gene ID: 64425
Uniprot ID: RPA49_HUMAN
Immunogen Region: 1-419
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
Immunogen Sequence: MAAEVLPSARWQYCGAPDGS QRAVLVQFSNGKLQSPGNMR FTLYENKDSTNPRKRNQRIL AAETDRLSYVGNNFGTGALK CNTLCRHFVGILNKTSGQME VYDAELFNMQPLFSDVSVES ELALESQTKTYREKMDSCIE AFGTTKQKRALNTRRMNRVG NESLNRAVAKAAETIIDTKG VTALVSDAIHNDLQDDSLYL PPCYDDAAKPEDVYKFEDLL SPAEYEALQSPSEAFRNVT
Post Translational Modifications Acetylated at Lys-373 by CREBBP/CBP, leading to decreased RNA polymerase I transcription. In normal conditions, deacetylated by SIRT7, promoting the association of RNA polymerase I with the rDNA promoter region and coding region. In response to stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased association of RNA polymerase I with the rDNA promoter region.
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Protein Name Dna-Directed Rna Polymerase I Subunit Rpa49
Rna Polymerase I Subunit A49
Dna-Directed Rna Polymerase I Subunit E
Rna Polymerase I-Associated Factor 1
Rna Polymerase I-Associated Factor 53
Database Links Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73863
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerase I Subunit Rpa49 antibody
Anti-Rna Polymerase I Subunit A49 antibody
Anti-Dna-Directed Rna Polymerase I Subunit E antibody
Anti-Rna Polymerase I-Associated Factor 1 antibody
Anti-Rna Polymerase I-Associated Factor 53 antibody
Anti-POLR1E antibody
Anti-PAF53 antibody
Anti-PRAF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance