| Host: |
Rabbit |
| Applications: |
WB/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLR1E |
| Gene ID: |
64425 |
| Uniprot ID: |
RPA49_HUMAN |
| Immunogen Region: |
1-419 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1). |
| Immunogen Sequence: |
MAAEVLPSARWQYCGAPDGS QRAVLVQFSNGKLQSPGNMR FTLYENKDSTNPRKRNQRIL AAETDRLSYVGNNFGTGALK CNTLCRHFVGILNKTSGQME VYDAELFNMQPLFSDVSVES ELALESQTKTYREKMDSCIE AFGTTKQKRALNTRRMNRVG NESLNRAVAKAAETIIDTKG VTALVSDAIHNDLQDDSLYL PPCYDDAAKPEDVYKFEDLL SPAEYEALQSPSEAFRNVT |
| Post Translational Modifications | Acetylated at Lys-373 by CREBBP/CBP, leading to decreased RNA polymerase I transcription. In normal conditions, deacetylated by SIRT7, promoting the association of RNA polymerase I with the rDNA promoter region and coding region. In response to stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased association of RNA polymerase I with the rDNA promoter region. |
| Function | Component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. |
| Protein Name | Dna-Directed Rna Polymerase I Subunit Rpa49Rna Polymerase I Subunit A49Dna-Directed Rna Polymerase I Subunit ERna Polymerase I-Associated Factor 1Rna Polymerase I-Associated Factor 53 |
| Database Links | Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73863 |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase I Subunit Rpa49 antibodyAnti-Rna Polymerase I Subunit A49 antibodyAnti-Dna-Directed Rna Polymerase I Subunit E antibodyAnti-Rna Polymerase I-Associated Factor 1 antibodyAnti-Rna Polymerase I-Associated Factor 53 antibodyAnti-POLR1E antibodyAnti-PAF53 antibodyAnti-PRAF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
