Western blot analysis of various lysates using POLE4 Rabbit pAb (STJ111924) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-POLE4 antibody (1-117) (STJ111924)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111924
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit polyclonal POLE4 (1-117) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPOLE4
Gene ID56655
Uniprot IDDPOE4_HUMAN
Immunogen Region1-117
Immunogen SequenceMAAAAAAGSGTPREEEGPAG EAAASQPQAPTSVPGARLSR LPLARVKALVKADPDVTLAG QEAIFILARAAELFVETIAK DAYCCAQQGKRKTLQRRDLD NAIEAVDEFAFLEGTLD
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLE4 (NP_063949.2).

Additional Info

Function Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication.
Protein Name Dna Polymerase Epsilon Subunit 4
Dna Polymerase Ii Subunit 4
Dna Polymerase Epsilon Subunit P12
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-5651801
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-68952
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Polymerase Epsilon Subunit 4 antibody
Anti-Dna Polymerase Ii Subunit 4 antibody
Anti-Dna Polymerase Epsilon Subunit P12 antibody
Anti-POLE4 antibody

Information sourced from Uniprot.org

Citations

Product Review

!