![Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101832/390042/STJ28552_1__58226.1713159925.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of various lysates using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101832/390043/STJ28552_2__10130.1713159926.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101832/390042/STJ28552_1__58226.1713159925.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLE3 |
| Gene ID: | 54107 |
| Uniprot ID: | DPOE3_HUMAN |
| Immunogen Region: | 1-147 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3). |
| Immunogen Sequence: | MAERPEDLNLPNAVITRIIK EALPDGVNISKEARSAISRA ASVFVLYATSCANNFAMKGK RKTLNASDVLSAMEEMEFQR FVTPLKEALEAYRREQKGKK EASEQKKKDKDKKTDSEEQD KSRDEDNDEDEERLEEEEQN EEEEVDN |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Function | Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication. Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex. |
| Protein Name | Dna Polymerase Epsilon Subunit 3 Arsenic-Transactivated Protein Astp Chromatin Accessibility Complex 17 Kda Protein Chrac-17 Huchrac17 Dna Polymerase Ii Subunit 3 Dna Polymerase Epsilon Subunit P17 |
| Database Links | Reactome: R-HSA-110314 Reactome: R-HSA-5651801 Reactome: R-HSA-5656169 Reactome: R-HSA-5685942 Reactome: R-HSA-5696397 Reactome: R-HSA-5696400 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-68952 Reactome: R-HSA-68962 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Polymerase Epsilon Subunit 3 antibody Anti-Arsenic-Transactivated Protein antibody Anti-Astp antibody Anti-Chromatin Accessibility Complex 17 Kda Protein antibody Anti-Chrac-17 antibody Anti-Huchrac17 antibody Anti-Dna Polymerase Ii Subunit 3 antibody Anti-Dna Polymerase Epsilon Subunit P17 antibody Anti-POLE3 antibody Anti-CHRAC17 antibody |
Information sourced from Uniprot.org