| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PNPLA2 (45-257) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PNPLA2 |
| Gene ID: |
57104 |
| Uniprot ID: |
PLPL2_HUMAN |
| Immunogen Region: |
45-257 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 45-257 of human PNPLA2 (NP_065109.1). |
| Immunogen Sequence: |
GASAGALTATALVTGVCLGE AGAKFIEVSKEARKRFLGPL HPSFNLVKIIRSFLLKVLPA DSHEHASGRLGISLTRVSDG ENVIISHFNSKDELIQANVC SGFIPVYCGLIPPSLQGVRY VDGGISDNLPLYELKNTITV SPFSGESDICPQDSSTNIHE LRVTNTSIQFNLRNLYRLSK ALFPPEPLVLREMCKQGYRD GLRFLQRNGLLNR |
| Tissue Specificity | Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity. Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation. Ubiquitinated by PEX2 in response to reactive oxygen species (ROS), leading to its degradation. |
| Function | Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Also possesses acylglycerol transacylase and phospholipase A2 activities. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes. |
| Protein Name | Patatin-Like Phospholipase Domain-Containing Protein 2Adipose Triglyceride LipaseCalcium-Independent Phospholipase A2-ZetaIpla2-ZetaDesnutrinPigment Epithelium-Derived Factor ReceptorPedf-RTts2.2Transport-Secretion Protein 2Tts2 |
| Database Links | Reactome: R-HSA-1482883Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Lipid DropletCell MembraneMulti-Pass Membrane ProteinCytoplasm |
| Alternative Antibody Names | Anti-Patatin-Like Phospholipase Domain-Containing Protein 2 antibodyAnti-Adipose Triglyceride Lipase antibodyAnti-Calcium-Independent Phospholipase A2-Zeta antibodyAnti-Ipla2-Zeta antibodyAnti-Desnutrin antibodyAnti-Pigment Epithelium-Derived Factor Receptor antibodyAnti-Pedf-R antibodyAnti-Tts2.2 antibodyAnti-Transport-Secretion Protein 2 antibodyAnti-Tts2 antibodyAnti-PNPLA2 antibodyAnti-ATGL antibodyAnti-FP17548 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
