• Immunofluorescence analysis of U2OS cells using PNLIP antibody (STJ29557). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using PNLIP antibody (STJ29557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of BxPC-3 cells, using PNLIP antibody (STJ29557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-PNLIP antibody (166-465) (STJ29557)

SKU:
STJ29557

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNLIP (166-465) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNLIP
Gene ID: 5406
Uniprot ID: LIPP_HUMAN
Immunogen Region: 166-465
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 166-465 of human PNLIP (NP_000927.1).
Immunogen Sequence: IGHSLGAHAAGEAGRRTNGT IGRITGLDPAEPCFQGTPEL VRLDPSDAKFVDVIHTDGAP IVPNLGFGMSQVVGHLDFFP NGGVEMPGCKKNILSQIVDI DGIWEGTRDFAACNHLRSYK YYTDSIVNPDGFAGFPCASY NVFTANKCFPCPSGGCPQMG HYADRYPGKTNDVGQKFYLD TGDASNFARWRYKVSVTLSG KKVTGHILVSLFGNKGNSKQ YEIFKGTLKPDSTHSNEFD
Function Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
Protein Name Pancreatic Triacylglycerol Lipase
Pl
Ptl
Pancreatic Lipase
Database Links Reactome: R-HSA-192456
Reactome: R-HSA-975634
Cellular Localisation Secreted
Alternative Antibody Names Anti-Pancreatic Triacylglycerol Lipase antibody
Anti-Pl antibody
Anti-Ptl antibody
Anti-Pancreatic Lipase antibody
Anti-PNLIP antibody

Information sourced from Uniprot.org

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