• Western blot analysis of various lysates using PMF1 Rabbit monoclonal antibody (STJ11104199) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using PMF1 Rabbit monoclonal antibody (STJ11104199) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PMF1 antibody (1-205) [S4199RM] (STJ11104199)

SKU:
STJ11104199

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Polyamine-Modulated Factor 1 (1-205) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4199RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PMF1
Gene ID: 100527963/11243
Uniprot ID: PMF1_HUMAN
Immunogen Region: 1-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PMF1 (NP_009152.2).
Immunogen Sequence: MAEASSANLGSGCEEKRHEG SSSESVPPGTTISRVKLLDT MVDTFLQKLVAAGSYQRFTD CYKCFYQLQPAMTQQIYDKF IAQLQTSIREEISDIKEEGN LEAVLNALDKIVEEGKVRKE PAWRPSGIPEKDLHSVMAPY FLQQRDTLRRHVQKQEAENQ QLADAVLAGRRQVEELQLQV QAQQQAWQALHREQRELVAV LREPE
Tissue Specificity Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver.
Function Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Protein Name Polyamine-Modulated Factor 1
Pmf-1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Associated With The Kinetochore
Alternative Antibody Names Anti-Polyamine-Modulated Factor 1 antibody
Anti-Pmf-1 antibody
Anti-PMF1 antibody

Information sourced from Uniprot.org

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