Anti-Pmel17/gp100 antibody [EBS-O-147] (STJ16101974)

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STJ16101974

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Host: Mouse
Applications: IF/IHC
Reactivity: Human/Horse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Pmel17/gp100 is suitable for use in Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: EBS-O-147
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Immunofluorescence on fixed cells (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minut
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: PMEL
Gene ID: 6490
Uniprot ID: PMEL_HUMAN
Immunogen: A BALB/c mouse was immunized with fresh metatstatic melanotic melanoma cell membranes. Fusion partner: SP-2/0.
Post Translational Modifications N- and O-glycosylated. A small amount of P1/P100 (major form) undergoes glycosylation in ER and Golgi compartments to yield P2/P120 (minor form). The mature P2 form leaves the trans-Golgi network and is mainly targeted to stage I melanosomes via the plasma membrane and clathrin-mediated endocytosis. Stage II melanosomes harbor only Golgi-modified fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides. O-glycosylation of the RPT region is a conserved feature likely involved in amyloid sheet separation via electrostatic repulsion. Undergoes multiple proteolytic processing. In a post-Golgi prelysosomal compartment, P2 is cleaved by a furin-like proprotein convertase (PC) into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, as most M-alpha and M-beta remain associated with each other intracellularly via a disulfide bond. Once targeted to stage I melanosomes, beta-secretase BACE2 cleaves the M-beta fragment to release the amyloidogenic luminal fragment containing M-alpha and a small portion of M-beta N-terminus. M-alpha is further cleaved by metalloproteinases, likely ADAM10 or ADAM17, and still unknown proteases to yield subfragments that ultimately assemble into amyloid fibrils. The C-terminal fragment of M-beta is processed by the gamma-secretase complex to release a short intracytoplasmic domain.
Function Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
Protein Name Melanocyte Protein Pmel
Me20-M
Me20m
Melanocyte Protein Pmel 17
Melanocytes Lineage-Specific Antigen Gp100
Melanoma-Associated Me20 Antigen
P1
P100
Premelanosome Protein
Silver Locus Protein Homolog Cleaved Into - M-Alpha
95 Kda Melanocyte-Specific Secreted Glycoprotein
P26
Secreted Melanoma-Associated Me20 Antigen
Me20-S
Me20s - M-Beta
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Cis-Golgi Network Membrane
Endosome
Multivesicular Body
Melanosome
Extracellular Vesicle
Secreted
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes Predominantly To Intralumenal Vesicles (Ilvs) Within Multivesicular Bodies
Associates With Ilvs Found Within The Lumen Of Premelanosomes And Melanosomes And Particularly In Compartments That Serve As Precursors To The Striated Stage Ii Premelanosomes
Sorted To Stage I Melanosomes Following Its Processing In The Er And Cis-Golgi
Transiently Expressed At The Cell Surface Before Targeting To Early Melanosomes
Colocalizes With Bace2 In Stage I And Ii Melanosomes
Colocalizes With Cd63 And Apoe At Exosomes And In Intraluminal Vesicles Within Multivesicular Endosomes
Alternative Antibody Names Anti-Melanocyte Protein Pmel antibody
Anti-Me20-M antibody
Anti-Me20m antibody
Anti-Melanocyte Protein Pmel 17 antibody
Anti-Melanocytes Lineage-Specific Antigen Gp100 antibody
Anti-Melanoma-Associated Me20 Antigen antibody
Anti-P1 antibody
Anti-P100 antibody
Anti-Premelanosome Protein antibody
Anti-Silver Locus Protein Homolog Cleaved Into - M-Alpha antibody
Anti-95 Kda Melanocyte-Specific Secreted Glycoprotein antibody
Anti-P26 antibody
Anti-Secreted Melanoma-Associated Me20 Antigen antibody
Anti-Me20-S antibody
Anti-Me20s - M-Beta antibody
Anti-PMEL antibody
Anti-D12S53E antibody
Anti-PMEL17 antibody
Anti-SILV antibody

Information sourced from Uniprot.org

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