• Western blot analysis of extracts of various cell lines, using PMAIP1 antibody (STJ111843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of mouse thymus cells using PMAIP1 antibody (STJ111843) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using PMAIP1 antibody (STJ111843) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat thymus cells using PMAIP1 antibody (STJ111843) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat thymus cells using PMAIP1 antibody (STJ111843) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using PMAIP1 antibody (STJ111843) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse thymus cells using PMAIP1 antibody (STJ111843) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using PMAIP1 Rabbit polyclonal antibody (STJ111843) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PMAIP1 antibody (1-54) (STJ111843)

SKU:
STJ111843

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PMAIP1 (1-54) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PMAIP1
Gene ID: 5366
Uniprot ID: APR_HUMAN
Immunogen Region: 1-54
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-54 of human PMAIP1 (NP_066950.1).
Immunogen Sequence: MPGKKARKNAQPSPARAPAE LEVECATQLRRFGDKLNFRQ KLLNLISKLFCSGT
Tissue Specificity Highly expressed in adult T-cell leukemia cell line.
Function Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
Protein Name Phorbol-12-Myristate-13-Acetate-Induced Protein 1
Pma-Induced Protein 1
Immediate-Early-Response Protein Apr
Protein Noxa
Database Links Reactome: R-HSA-111448
Reactome: R-HSA-111453
Reactome: R-HSA-6803204
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Phorbol-12-Myristate-13-Acetate-Induced Protein 1 antibody
Anti-Pma-Induced Protein 1 antibody
Anti-Immediate-Early-Response Protein Apr antibody
Anti-Protein Noxa antibody
Anti-PMAIP1 antibody
Anti-NOXA antibody

Information sourced from Uniprot.org

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