• Western blot analysis of extracts of various cell lines, using PLS3 antibody (STJ25032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PLS3 antibody (STJ25032). Western blot was performed from the immunoprecipitate using PLS3 antibody (STJ25032) at a dilition of 1:1000.

Anti-PLS3 antibody (1-240) (STJ25032)

SKU:
STJ25032

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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLS3
Gene ID: 5358
Uniprot ID: PLST_HUMAN
Immunogen Region: 1-240
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2).
Immunogen Sequence: MDEMATTQISKDELDELKEA FAKVDLNSNGFICDYELHEL FKEANMPLPGYKVREIIQKL MLDGDRNKDGKISFDEFVYI FQEVKSSDIAKTFRKAINRK EGICALGGTSELSSEGTQHS YSEEEKYAFVNWINKALEND PDCRHVIPMNPNTDDLFKAV GDGIVLCKMINLSVPDTIDE RAINKKKLTPFIIQENLNLA LNSASAIGCHVVNIGAEDLR AGKPHLVLGLLWQIIKIGL
Tissue Specificity Expressed in a variety of organs, including muscle, brain, uterus and esophagus.
Function Actin-bundling protein.
Protein Name Plastin-3
T-Fimbrin
T-Plastin
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Plastin-3 antibody
Anti-T-Fimbrin antibody
Anti-T-Plastin antibody
Anti-PLS3 antibody

Information sourced from Uniprot.org

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