Anti-PLAT antibody (463-562) [S7MR] (STJ11102017)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal PLAT (463-562) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IF/ICC:1:100-1:400ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLAT |
| Gene ID | 5327 |
| Uniprot ID | TPA_HUMAN |
| Immunogen Region | 463-562 |
| Immunogen Sequence | LKEAHVRLYPSSRCTSQHLL NRTVTDNMLCAGDTRSGGPQ ANLHDACQGDSGGPLVCLND GRMTLVGIISWGLGCGQKDV PGVYTKVTNYLDWIRDNMRP |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 463-562 of human tPA/tPA/PLAT (P00750). |
Additional Info
| Tissue Specificity | Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. |
| Post Translational Modifications | The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin. N-glycosylation of Asn-152.the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. Characterization of O-linked glycan was studied in Bowes melanoma cell line. |
| Function | Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy. |
| Protein Name | Tissue-Type Plasminogen ActivatorT-PaT-Plasminogen ActivatorTpaAlteplaseReteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-186797Reactome: R-HSA-75205 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Tissue-Type Plasminogen Activator antibodyAnti-T-Pa antibodyAnti-T-Plasminogen Activator antibodyAnti-Tpa antibodyAnti-Alteplase antibodyAnti-Reteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B antibodyAnti-PLAT antibody |
Information sourced from Uniprot.org