• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PJVK, using PJVK Rabbit monoclonal antibody (STJ11105209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-PJVK antibody (290-352) [S5209RM] (STJ11105209)

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STJ11105209

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Pejvakin (290-352) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5209RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PJVK
Gene ID: 494513
Uniprot ID: PJVK_HUMAN
Immunogen Region: 290-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-352 of human PJVK (NP_001036167.1).
Immunogen Sequence: KEGTHIRVNLLNHNIPKGPC ILCGMGNFKRETVYGCFQCS VDGQKYVRLHAVPCFDIWHK RMK
Function Peroxisome-associated protein required to protect auditory hair cells against noise-induced damage. Acts by regulating noise-induced peroxisome proliferation in auditory hair cells and neurons, and promoting autophagic degradation of damaged peroxisomes (pexophagy). Noise overexposure increases reactive oxygen species (ROS) levels, causing oxidative damage to auditory hair cells and resulting in hearing loss. PJVK acts as a ROS sensor that recruits the autophagy machinery to trigger pexophagy of peroxisomes damaged by oxidative stress. In addition to pexophagy, also required to promote peroxisome proliferation in response to sound overstimulation.
Protein Name Pejvakin
Autosomal Recessive Deafness Type 59 Protein
Database Links Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Peroxisome Membrane
Cell Projection
Cilium
Associates With The Peroxisomal Membrane
It Is Unclear Whether It Is Embedded Or Just Associated With The Peroxisomal Membrane
Localizes To Ciliary Rootlet
Alternative Antibody Names Anti-Pejvakin antibody
Anti-Autosomal Recessive Deafness Type 59 Protein antibody
Anti-PJVK antibody
Anti-DFNB59 antibody

Information sourced from Uniprot.org

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