• Western blot analysis of various lysates using PISD Rabbit polyclonal antibody (STJ116249) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PISD antibody (1-375) (STJ116249)

SKU:
STJ116249

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PISD (1-375) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PISD
Gene ID: 23761
Uniprot ID: PISD_HUMAN
Immunogen Region: 1-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human PISD (NP_055153.1).
Immunogen Sequence: MMCQSEARQGPELRAAKWLH FPQLALRRRLGQLSCMSRPA LKLRSWPLTVLYYLLPFGAL RPLSRVGWRPVSRVALYKSV PTRLLSRAWGRLNQVELPHW LRRPVYSLYIWTFGVNMKEA AVEDLHHYRNLSEFFRRKLK PQARPVCGLHSVISPSDGRI LNFGQVKNCEVEQVKGVTYS LESFLGPRMCTEDLPFPPAA SCDSFKNQLVTREGNELYHC VIYLAPGDYHCFHSPTDWT
Post Translational Modifications Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.
Function Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane.
Protein Name Phosphatidylserine Decarboxylase Proenzyme - Mitochondrial Cleaved Into - Phosphatidylserine Decarboxylase Beta Chain - Phosphatidylserine Decarboxylase Alpha Chain
Database Links Reactome: R-HSA-1483213
Cellular Localisation Phosphatidylserine Decarboxylase Beta Chain: Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Intermembrane Side
Phosphatidylserine Decarboxylase Alpha Chain: Mitochondrion Inner Membrane
Peripheral Membrane Protein
Cytoplasm
Anchored To The Mitochondrial Inner Membrane Through Its Interaction With The Integral Membrane Beta Chain
Isoform 1: Mitochondrion Inner Membrane
Isoform 2: Mitochondrion Inner Membrane
Lipid Droplet
Predominantly Localizes To Lipid Droplets In Lipid-Replete Conditions
And To Mitochondria In Lipid-Deplete Conditions
Alternative Antibody Names Anti-Phosphatidylserine Decarboxylase Proenzyme - Mitochondrial Cleaved Into - Phosphatidylserine Decarboxylase Beta Chain - Phosphatidylserine Decarboxylase Alpha Chain antibody
Anti-PISD antibody

Information sourced from Uniprot.org

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