• Western blot analysis of extracts of various cell lines, using PIN4 antibody (STJ11104113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIN4 antibody (52-131) [S4113RM] (STJ11104113)

SKU:
STJ11104113

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 4 (52-131) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4113RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIN4
Gene ID: 5303
Uniprot ID: PIN4_HUMAN
Immunogen Region: 52-131
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 52-131 of human PIN4 (Q9Y237).
Immunogen Sequence: MEAMEKLKSGMRFNEVAAQY SEDKARQGGDLGWMTRGSMV GPFQEAAFALPVSGMDKPVF TDPPVKTKFGYHIIMVEGRK
Tissue Specificity Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.
Post Translational Modifications Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus. Isoform 2 is sumoylated with SUMO2 and SUMO3.
Function Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Isoform 2 binds to double-stranded DNA.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 4
Parvulin-14
Par14
Hpar14
Parvulin-17
Par17
Hpar17
Peptidyl-Prolyl Cis-Trans Isomerase Pin4
Ppiase Pin4
Peptidyl-Prolyl Cis/Trans Isomerase Epvh
Hepvh
Rotamase Pin4
Cellular Localisation Isoform 1: Nucleus
Nucleolus
Cytoplasm
Cytoskeleton
Spindle
Colocalizes In The Nucleolus During Interphase And On The Spindle Apparatus During Mitosis With Npm1
Isoform 2: Mitochondrion
Mitochondrion Matrix
Imported In A Time- And Membrane Potential-Dependent Manner To The Mitochondrial Matrix
But Without Concomitant Processing Of The Protein
Directed To Mitochondria By A Novel N-Terminal Domain That Functions As Non-Cleavable Mitochondrial Targeting Peptide
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 4 antibody
Anti-Parvulin-14 antibody
Anti-Par14 antibody
Anti-Hpar14 antibody
Anti-Parvulin-17 antibody
Anti-Par17 antibody
Anti-Hpar17 antibody
Anti-Peptidyl-Prolyl Cis-Trans Isomerase Pin4 antibody
Anti-Ppiase Pin4 antibody
Anti-Peptidyl-Prolyl Cis/Trans Isomerase Epvh antibody
Anti-Hepvh antibody
Anti-Rotamase Pin4 antibody
Anti-PIN4 antibody

Information sourced from Uniprot.org

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