| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PIK3CA (401-1068) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIK3CA |
| Gene ID: |
5290 |
| Uniprot ID: |
PK3CA_HUMAN |
| Immunogen Region: |
401-1068 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 401-1068 of human PIK3CA (NP_006209.2). |
| Immunogen Sequence: |
RLCLSICSVKGRKGAKEEHC PLAWGNINLFDYTDTLVSGK MALNLWPVPHGLEDLLNPIG VTGSNPNKETPCLELEFDWF SSVVKFPDMSVIEEHANWSV SREAGFSYSHAGLSNRLARD NELRENDKEQLKAISTRDPL SEITEQEKDFLWSHRHYCVT IPEILPKLLLSVKWNSRDEV AQMYCLVKDWPPIKPEQAME |
| Function | Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others. Plays a role in the positive regulation of phagocytosis and pinocytosis. |
| Protein Name | Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Alpha IsoformPi3-Kinase Subunit AlphaPi3k-AlphaPi3kalphaPtdins-3-Kinase Subunit AlphaPhosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit AlphaPtdins-3-Kinase Subunit P110-AlphaP110alphaPhosphoinositide 3-Kinase AlphaPhosphoinositide-3-Kinase Catalytic Alpha PolypeptideSerine/Threonine Protein Kinase Pik3ca |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-112399Reactome: R-HSA-114604Reactome: R-HSA-1236382Reactome: R-HSA-1250342Reactome: R-HSA-1257604Reactome: R-HSA-1433557Reactome: R-HSA-1660499Reactome: R-HSA-180292Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-1963642Reactome: R-HSA-198203Reactome: R-HSA-201556Reactome: R-HSA-202424Reactome: R-HSA-2029485Reactome: R-HSA-210993Reactome: R-HSA-2219530Reactome: R-HSA-2424491Reactome: R-HSA-2730905Reactome: R-HSA-373753Reactome: R-HSA-388841Reactome: R-HSA-389357Reactome: R-HSA-416476Reactome: R-HSA-4420097Reactome: R-HSA-512988Reactome: R-HSA-5637810Reactome: R-HSA-5654689Reactome: R-HSA-5654695Reactome: R-HSA-5654710Reactome: R-HSA-5654720Reactome: R-HSA-5655253Reactome: R-HSA-5655291Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-8851907Reactome: R-HSA-8853659Reactome: R-HSA-9009391Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9027276Reactome: R-HSA-9028335Reactome: R-HSA-912526Reactome: R-HSA-912631Reactome: R-HSA-9603381Reactome: R-HSA-9607240Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9670439Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-9680350Reactome: R-HSA-9703465Reactome: R-HSA-9703648Reactome: R-HSA-9725370 |
| Alternative Antibody Names | Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform antibodyAnti-Pi3-Kinase Subunit Alpha antibodyAnti-Pi3k-Alpha antibodyAnti-Pi3kalpha antibodyAnti-Ptdins-3-Kinase Subunit Alpha antibodyAnti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Alpha antibodyAnti-Ptdins-3-Kinase Subunit P110-Alpha antibodyAnti-P110alpha antibodyAnti-Phosphoinositide 3-Kinase Alpha antibodyAnti-Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide antibodyAnti-Serine/Threonine Protein Kinase Pik3ca antibodyAnti-PIK3CA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
