• Western blot analysis of extracts of various cell lines, using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded rat testis tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat testis using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded human colon tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human appendix using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded rat colon tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded mouse heart tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded mouse colon tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded human liver tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded human thyroid tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIAS1/PIAS2 antibody (552-651) [S4MR] (STJ11102524)

SKU:
STJ11102524

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIAS2
PIAS1
Gene ID: 9063
8554
Uniprot ID: PIAS2_HUMAN
PIAS1_HUMAN
Immunogen Region: 552-651
Specificity: A synthetic peptide corresponding to a sequence within amino acids 552-651 of human PIAS1+PIAS2 (O75925).
Immunogen Sequence: DNQHYNTSLLAAAAAAVSDD QDLLHSSRFFPYTSSQMFLD QLSAGGSTSLPTTNGSSSGS NSSLVSSNSLRESHSHTVTN RSSTDTASIFGIIPDIISLD
Tissue Specificity O75928 - Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
O75925 - Expressed in numerous tissues with highest level in testis.
Alternative Antibody Names Anti-E3 Sumo-Protein Ligase Pias2 antibody
Anti-Androgen Receptor-Interacting Protein 3 antibody
Anti-Arip3 antibody
Anti-Dab2-Interacting Protein antibody
Anti-Dip antibody
Anti-E3 Sumo-Protein Transferase Pias2 antibody
Anti-Msx-Interacting Zinc Finger Protein antibody
Anti-Miz1 antibody
Anti-Pias-Ny Protein antibody
Anti-Protein Inhibitor Of Activated Stat X antibody
Anti-Protein Inhibitor Of Activated Stat2 antibody
Anti-PIAS2 antibody
Anti-PIASX antibody
Anti-E3 Sumo-Protein Ligase Pias1 antibody
Anti-Dead/H Box-Binding Protein 1 antibody
Anti-E3 Sumo-Protein Transferase Pias1 antibody
Anti-Gu-Binding Protein antibody
Anti-Gbp antibody
Anti-Protein Inhibitor Of Activated Stat Protein 1 antibody
Anti-Rna Helicase Ii-Binding Protein antibody
Anti-PIAS1 antibody
Anti-DDXBP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance