• Western blot analysis of lysates from HepG2 cells, using WASP (Phospho-Tyr290) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using WASP (Phospho-Tyr290) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-WAS-Tyr290 antibody (256-305 aa) (STJ90839)

SKU:
STJ90839

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Actin nucleation-promoting factor WAS-Tyr290 (256-305 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WAS
Gene ID: 7454
Uniprot ID: WASP_HUMAN
Immunogen Region: 256-305 aa
Specificity: Phospho-WASP (Y290) Polyclonal Antibody detects endogenous levels of WASP protein only when phosphorylated at Y290.
Immunogen: The antiserum was produced against synthesized peptide derived from the human WASP around the phosphorylation site of Tyr290 at the amino acid range 256-305
Post Translational Modifications Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.
Function Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Protein Name Actin Nucleation-Promoting Factor Was
Wiskott-Aldrich Syndrome Protein
Wasp
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-2029482
Reactome: R-HSA-5663213
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013409
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Alternative Antibody Names Anti-Actin Nucleation-Promoting Factor Was antibody
Anti-Wiskott-Aldrich Syndrome Protein antibody
Anti-Wasp antibody
Anti-WAS antibody
Anti-IMD2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance