• Western blot analysis of extracts from 293 cells, using Phospho-TP73-Y99 antibody (STJ22442). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of MCF7, using Phospho-TP73-Y99 Rabbit polyclonal antibody (STJ22442) at 1:500 dilution. MCF7 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-Phospho-TP73-Y99 antibody (STJ22442)

SKU:
STJ22442

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-TP73-Y99 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TP73
Gene ID: 7161
Uniprot ID: P73_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding Y99 of human TP73
Immunogen Sequence: Email for sequence
Tissue Specificity Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. Expressed in the respiratory epithelium.
Post Translational Modifications Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Polyubiquitinated by RCHY1/PIRH2.leading to its degradation by the proteasome.
Function Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression. It is an essential factor for the positive regulation of lung ciliated cell differentiation.
Protein Name Tumor Protein P73
P53-Like Transcription Factor
P53-Related Protein
Database Links Reactome: R-HSA-139915
Reactome: R-HSA-6803204
Reactome: R-HSA-6803205
Reactome: R-HSA-6803207
Reactome: R-HSA-6803211
Reactome: R-HSA-6804759
Reactome: R-HSA-8939236
Cellular Localisation Nucleus
Cytoplasm
Accumulates In The Nucleus In Response To Dna Damage
Alternative Antibody Names Anti-Tumor Protein P73 antibody
Anti-P53-Like Transcription Factor antibody
Anti-P53-Related Protein antibody
Anti-TP73 antibody
Anti-P73 antibody

Information sourced from Uniprot.org

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