| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Tumor protein p73-Tyr99 (66-115 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TP73 |
| Gene ID: | 7161 |
| Uniprot ID: | P73_HUMAN |
| Immunogen Region: | 66-115 aa |
| Specificity: | Phospho-p73 (Y99) Polyclonal Antibody detects endogenous levels of p73 protein only when phosphorylated at Y99. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human p73 around the phosphorylation site of Tyr99 at the amino acid range 66-115 |
| Post Translational Modifications | Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Polyubiquitinated by RCHY1/PIRH2.leading to its degradation by the proteasome. |
| Function | Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression. It is an essential factor for the positive regulation of lung ciliated cell differentiation. |
| Protein Name | Tumor Protein P73 P53-Like Transcription Factor P53-Related Protein |
| Database Links | Reactome: R-HSA-139915 Reactome: R-HSA-6803204 Reactome: R-HSA-6803205 Reactome: R-HSA-6803207 Reactome: R-HSA-6803211 Reactome: R-HSA-6804759 Reactome: R-HSA-8939236 |
| Cellular Localisation | Nucleus Cytoplasm Accumulates In The Nucleus In Response To Dna Damage |
| Alternative Antibody Names | Anti-Tumor Protein P73 antibody Anti-P53-Like Transcription Factor antibody Anti-P53-Related Protein antibody Anti-TP73 antibody Anti-P73 antibody |
Information sourced from Uniprot.org