| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
TIFA |
| Gene ID: |
92610 |
| Uniprot ID: |
TIFA_HUMAN |
| Specificity: |
A synthetic phosphorylated peptide around T9 of human TIFA (NP_443096.1).. |
| Immunogen Sequence: |
ADTEE |
| Post Translational Modifications | Phosphorylated at Thr-9 following detection of ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose) by ALPK1. Phosphorylation at Thr-9 by ALPK1 leads to the formation of an intermolecular binding between the FHA domain and phosphorylated Thr-9, promoting TIFA oligomerization and TIFA-mediated NF-kappa-B activation. |
| Function | Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling. |
| Protein Name | Traf-Interacting Protein With Fha Domain-Containing Protein APutative Mapk-Activating Protein Pm14Putative Nf-Kappa-B-Activating Protein 20Traf2-Binding Protein |
| Database Links | Reactome: R-HSA-445989Reactome: R-HSA-9645460 |
| Cellular Localisation | CytoplasmColocalizes With Lysosomal Marker Lamp2 Following Homooligomerization And Subsequent Activation |
| Alternative Antibody Names | Anti-Traf-Interacting Protein With Fha Domain-Containing Protein A antibodyAnti-Putative Mapk-Activating Protein Pm14 antibodyAnti-Putative Nf-Kappa-B-Activating Protein 20 antibodyAnti-Traf2-Binding Protein antibodyAnti-TIFA antibodyAnti-T2BP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
