Anti-Phospho-TIFA-T9 antibody (STJ11107723)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Phospho-TIFA-T9 antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TIFA |
| Gene ID | 92610 |
| Uniprot ID | TIFA_HUMAN |
| Immunogen Sequence | ADTEE |
| Specificity | A synthetic phosphorylated peptide around T9 of human TIFA (NP_443096.1).. |
Additional Info
| Post Translational Modifications | Phosphorylated at Thr-9 following detection of ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose) by ALPK1. Phosphorylation at Thr-9 by ALPK1 leads to the formation of an intermolecular binding between the FHA domain and phosphorylated Thr-9, promoting TIFA oligomerization and TIFA-mediated NF-kappa-B activation. |
| Function | Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling. |
| Protein Name | Traf-Interacting Protein With Fha Domain-Containing Protein APutative Mapk-Activating Protein Pm14Putative Nf-Kappa-B-Activating Protein 20Traf2-Binding Protein |
| Database Links | Reactome: R-HSA-445989Reactome: R-HSA-9645460 |
| Cellular Localisation | CytoplasmColocalizes With Lysosomal Marker Lamp2 Following Homooligomerization And Subsequent Activation |
| Alternative Antibody Names | Anti-Traf-Interacting Protein With Fha Domain-Containing Protein A antibodyAnti-Putative Mapk-Activating Protein Pm14 antibodyAnti-Putative Nf-Kappa-B-Activating Protein 20 antibodyAnti-Traf2-Binding Protein antibodyAnti-TIFA antibodyAnti-T2BP antibody |
Information sourced from Uniprot.org