• Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of L929 using p-TBC1D4 (T642) antibody. Antibody was diluted at 1:2000

Anti-Phospho-TBC1D4-Thr642 antibody (611-660 aa) (STJ91288)

SKU:
STJ91288

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-TBC1 domain family member 4-Thr642 (611-660 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBC1D4
Gene ID: 9882
Uniprot ID: TBCD4_HUMAN
Immunogen Region: 611-660 aa
Specificity: Phospho-TBC1D4 (T642) Polyclonal Antibody detects endogenous levels of TBC1D4 protein only when phosphorylated at T642.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AS160 around the phosphorylation site of Thr642 at the amino acid range 611-660
Post Translational Modifications Phosphorylated by AKT1.insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Function May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Protein Name Tbc1 Domain Family Member 4
Akt Substrate Of 160 Kda
As160
Database Links Reactome: R-HSA-1445148
Cellular Localisation Cytoplasm
Isoform 2 Shows A Cytoplasmic Perinuclear Localization In A Myoblastic Cell Line In Resting And Insulin-Stimulated Cells
Alternative Antibody Names Anti-Tbc1 Domain Family Member 4 antibody
Anti-Akt Substrate Of 160 Kda antibody
Anti-As160 antibody
Anti-TBC1D4 antibody
Anti-AS160 antibody
Anti-KIAA0603 antibody

Information sourced from Uniprot.org

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