• Western blot analysis of extracts of 293T cells, using Phospho-SMC1-S957 rabbit monoclonal antibody (STJ11102639) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of Phospho-SMC1-S957 in paraffin-embedded human small intestine tissue using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SMC1-S957 rabbit monoclonal antibody (STJ11102639) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of Phospho-SMC1-S957 in paraffin-embedded human esophagus tissue using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human colon using Phospho-SMC1-S957 rabbit monoclonal antibody (STJ11102639) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-SMC1-S957 in paraffin-embedded rat lung tissue using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using Phospho-SMC1-S957 rabbit monoclonal antibody (STJ11102639) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-SMC1-S957 in paraffin-embedded mouse kidney tissue using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat brain using Phospho-SMC1-S957 rabbit monoclonal antibody (STJ11102639) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-SMC1-S957 in paraffin-embedded mouse testis tissue using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-SMC1-S957 in paraffin-embedded human colon carcinoma tissue using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit monoclonal antibody (STJ11102639) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-SMC1A-S957 antibody [S9MR] (STJ11102639)

SKU:
STJ11102639

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-SMC1-S957 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMC1A
Gene ID: 8243
Uniprot ID: SMC1A_HUMAN
Immunogen: A synthetic phosphorylated peptide around S957 of human SMC1 (Q14683).
Immunogen Sequence: GSSQG
Post Translational Modifications Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation. Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
Function Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Protein Name Structural Maintenance Of Chromosomes Protein 1a
Smc Protein 1a
Smc-1-Alpha
Smc-1a
Sb1.8
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2467813
Reactome: R-HSA-2468052
Reactome: R-HSA-2470946
Reactome: R-HSA-2500257
Reactome: R-HSA-3108214
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Associates With Chromatin
Before Prophase It Is Scattered Along Chromosome Arms
During Prophase
Most Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By Plk
Except At Centromeres
Where Cohesin Complexes Remain
At Anaphase
The Rad21 Subunit Of The Cohesin Complex Is Cleaved
Leading To The Dissociation Of The Complex From Chromosomes
Allowing Chromosome Separation
In Germ Cells
Cohesin Complex Dissociates From Chromatin At Prophase I
And May Be Replaced By A Meiosis-Specific Cohesin Complex
The Phosphorylated Form On Ser-957 And Ser-966 Associates With Chromatin During G1/S/G2 Phases But Not During M Phase
Suggesting That Phosphorylation Does Not Regulate Cohesin Function
Integral Component Of The Functional Centromere-Kinetochore Complex At The Kinetochore Region During Mitosis
Alternative Antibody Names Anti-Structural Maintenance Of Chromosomes Protein 1a antibody
Anti-Smc Protein 1a antibody
Anti-Smc-1-Alpha antibody
Anti-Smc-1a antibody
Anti-Sb1.8 antibody
Anti-SMC1A antibody
Anti-DXS423E antibody
Anti-KIAA0178 antibody
Anti-SB1.8 antibody
Anti-SMC1 antibody
Anti-SMC1L1 antibody

Information sourced from Uniprot.org

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