• Western blot analysis of extracts of various cell lines, using Phospho-Smad2 (Ser465/467) /Smad3 (Ser423/425) antibody (STJ114840) at 1:1000 dilution. HeLa and C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of C2C12 cells, using IL2 antibody (STJ230068) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of HeLa cells, using IL2 antibody (STJ230068) at 1:1000 dilution. HeLa cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded Rat brain using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Human esophagus using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100 40x lens).
  • Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ114840) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Phospho-SMAD2/SMAD3-S423/425 antibody (STJ114840)

SKU:
STJ114840

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD3
SMAD2
Gene ID: 4088
4087
Uniprot ID: SMAD3_HUMAN
SMAD2_HUMAN
Specificity: A synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/467 + Smad3-S423/425SMAD2 (NP_005892.1).
Immunogen Sequence: RCSSMS
Tissue Specificity P84022 - NA
Q15796 - Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 3 antibody
Anti-Mad Homolog 3 antibody
Anti-Mad3 antibody
Anti-Mothers Against Dpp Homolog 3 antibody
Anti-Hmad-3 antibody
Anti-Jv15-2 antibody
Anti-Smad Family Member 3 antibody
Anti-Smad 3 antibody
Anti-Smad3 antibody
Anti-Hsmad3 antibody
Anti-SMAD3 antibody
Anti-MADH3 antibody
Anti-Mothers Against Decapentaplegic Homolog 2 antibody
Anti-Mad Homolog 2 antibody
Anti-Mothers Against Dpp Homolog 2 antibody
Anti-Jv18-1 antibody
Anti-Mad-Related Protein 2 antibody
Anti-Hmad-2 antibody
Anti-Smad Family Member 2 antibody
Anti-Smad 2 antibody
Anti-Smad2 antibody
Anti-Hsmad2 antibody
Anti-SMAD2 antibody
Anti-MADH2 antibody
Anti-MADR2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance