• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SKP2-Ser64 antibody (STJ196425)

SKU:
STJ196425

€57.50 - €282.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-S-phase kinase-associated protein 2-Ser64 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SKP2
Gene ID: 6502
Uniprot ID: SKP2_HUMAN
Specificity: This antibody detects endogenous levels of Human Skp2 (phospho-Ser64)
Immunogen: Synthesized phosho peptide around human Skp2 (Ser64)
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
Protein Name S-Phase Kinase-Associated Protein 2
Cyclin-A/Cdk2-Associated Protein P45
F-Box Protein Skp2
F-Box/Lrr-Repeat Protein 1
P45skp2
Database Links Reactome: R-HSA-174178
Reactome: R-HSA-187577
Reactome: R-HSA-5689880
Reactome: R-HSA-68949
Reactome: R-HSA-69231
Reactome: R-HSA-8939902
Reactome: R-HSA-8951664
Reactome: R-HSA-9687136
Reactome: R-HSA-9708530
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-S-Phase Kinase-Associated Protein 2 antibody
Anti-Cyclin-A/Cdk2-Associated Protein P45 antibody
Anti-F-Box Protein Skp2 antibody
Anti-F-Box/Lrr-Repeat Protein 1 antibody
Anti-P45skp2 antibody
Anti-SKP2 antibody
Anti-FBXL1 antibody

Information sourced from Uniprot.org

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