• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SGO1-Ser14 antibody (1-50 aa) (STJ91267)

SKU:
STJ91267

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Shugoshin 1-Ser14 (1-50 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGO1
Gene ID: 151648
Uniprot ID: SGO1_HUMAN
Immunogen Region: 1-50 aa
Specificity: Phospho-Sgo1 (S14) Polyclonal Antibody detects endogenous levels of Sgo1 protein only when phosphorylated at S14.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SGO1 around the phosphorylation site of Ser14 at the amino acid range 1-50
Post Translational Modifications Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1. Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB.
Function Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.
Protein Name Shugoshin 1
Serologically Defined Breast Cancer Antigen Ny-Br-85
Shugoshin-Like 1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Cytoplasm
Cytoskeleton
Spindle Pole
Microtubule Organizing Center
Centrosome
Localizes To The Inner Centromere Throughout Prophase Until Metaphase And Disappears At Anaphase
Centromeric Localization Requires The Presence Of Bub1 And The Interaction With Ppp2r1a
Colocalizes With Nek2 At The Kinetochore
Colocalizes With And Ss18l1 At The Kinetochore
Phosphorylation By Aukrb And The Presence Of Bub1 Are Required For Localization To The Kinetochore
Isoform 1 Primarily Localizes To Kinetochores During G2 Phase And Mitotic Prophase
Metaphase
And Anaphase And Does Not Appear To Be Associated With Kinetochores During Late Mitosis
Isoform 3 Is Found At The Centrosome In Interphase And At Spindle Poles In Mitosis And Its Spindle Pole Localization Is Plk1 Dependent
Isoform 3 Does Not Localize To Kinetochores During Any Stages Of The Cell Cycle
Alternative Antibody Names Anti-Shugoshin 1 antibody
Anti-Serologically Defined Breast Cancer Antigen Ny-Br-85 antibody
Anti-Shugoshin-Like 1 antibody
Anti-SGO1 antibody
Anti-SGOL1 antibody

Information sourced from Uniprot.org

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