• Western blot of Jurkat cell lysate showing specific immunolabeling of the ~28 kDa rpS6 phosphorylated at Ser244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-RPS6-Ser244 antibody (STJA0003772)

SKU:
STJA0003772-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Chicken/Goat/Non-Human Primates/Rat/Xenopus/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Ribosomal protein S6-Ser244 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:500
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Rps6
Gene ID: 20104
Uniprot ID: RS6_MOUSE
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH.
Post Translational Modifications Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3.phosphorylation at these sites facilitates the assembly of the pre-initiation complex. Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8. Mono-ADP-ribosylation at Glu-35 by PARP16 inhibits polysome assembly and mRNA loading, thereby inhibiting protein translation.
Function Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Es6
40s Ribosomal Protein S6
Phosphoprotein Np33
Database Links Reactome: R-MMU-156827
Reactome: -MMU-166208
Reactome: -MMU-1799339
Reactome: -MMU-6791226
Reactome: -MMU-72649
Reactome: -MMU-72689
Reactome: -MMU-72695
Reactome: -MMU-72702
Reactome: -MMU-72706
Reactome: -MMU-975956
Reactome: -MMU-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es6 antibody
Anti-40s Ribosomal Protein S6 antibody
Anti-Phosphoprotein Np33 antibody
Anti-Rps6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance