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Anti-Phospho-RPS6-S240/244 antibody (STJ113488)

Western blot analysis of extracts of various cell lines, using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 antibody (STJ113488) at 1:1000 dilution or RPS6 antibody (A6058). HeLa cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g Phospho-S6 Ribosomal Protein (RPS6) -S240/244 polyclonal antibody (STJ113488). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 polyclonal antibody (STJ113488) at a dilution of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight.
Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 rabbit polyclonal antibody (STJ113488) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of Phospho-S6 Ribosomal Protein (RPS6) -S240/244 in paraffin-embedded rat brain using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit polyclonal antibody (STJ113488) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 rabbit polyclonal antibody (STJ113488) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of Phospho-S6 Ribosomal Protein (RPS6) -S240/244 in paraffin-embedded mouse pancreas using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit polyclonal antibody (STJ113488) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-S6 Ribosomal Protein (RPS6) -S240/244 polyclonal antibody (STJ113488). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 polyclonal antibody (STJ113488) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight.
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit polyclonal antibody (STJ113488) at 1:1000 dilution or RPS6 antibody (A6058). HeLa cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-RPS6-S240/244 antibody (STJ113488)

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STJ113488

£129.50 - £639.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IHC/IP
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-RPS6-S240/244 is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Purification:	Affinity purification

Gene Symbol:	RPS6
Gene ID:	6194
Uniprot ID:	RS6_HUMAN
Immunogen:	A synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2).
Immunogen Sequence:	STSKS

Post Translational Modifications	Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3.phosphorylation at these sites facilitates the assembly of the pre-initiation complex. Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8. Mono-ADP-ribosylation at Glu-35 by PARP16 inhibits polysome assembly and mRNA loading, thereby inhibiting protein translation.
Function	Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name	Small Ribosomal Subunit Protein Es6 40s Ribosomal Protein S6 Phosphoprotein Np33
Database Links	Reactome: R-HSA-156827 Reactome: R-HSA-156902 Reactome: R-HSA-166208 Reactome: R-HSA-1799339 Reactome: R-HSA-192823 Reactome: R-HSA-2408557 Reactome: R-HSA-6790901 Reactome: R-HSA-6791226 Reactome: R-HSA-72649 Reactome: R-HSA-72689 Reactome: R-HSA-72695 Reactome: R-HSA-72702 Reactome: R-HSA-72706 Reactome: R-HSA-72764 Reactome: R-HSA-9010553 Reactome: R-HSA-9633012 Reactome: R-HSA-9725371 Reactome: R-HSA-9735869 Reactome: R-HSA-9754678 Reactome: R-HSA-975956 Reactome: R-HSA-975957
Cellular Localisation	Cytoplasm Nucleus Nucleolus
Alternative Antibody Names	Anti-Small Ribosomal Subunit Protein Es6 antibody Anti-40s Ribosomal Protein S6 antibody Anti-Phosphoprotein Np33 antibody Anti-RPS6 antibody Anti-OK antibody Anti-SW-cl.2 antibody

Information sourced from Uniprot.org

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