• Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1/AMPKa2-T183/T172 antibody (STJ11102659) at 1:1000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. C6 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit polyclonal antibody (STJ11102659) at 1:1000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. C6 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-PRKAA1/PRKAA2-T183/T172 antibody (STJ11102659)

SKU:
STJ11102659

£129.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAA1
PRKAA2
Gene ID: 5562
5563
Uniprot ID: AAPK1_HUMAN
AAPK2_HUMAN
Specificity: A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5).
Immunogen Sequence: LRTSC
Tissue Specificity
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-1 antibody
Anti-Ampk Subunit Alpha-1 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-Tau-Protein Kinase Prkaa1 antibody
Anti-PRKAA1 antibody
Anti-AMPK1 antibody
Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-2 antibody
Anti-Ampk Subunit Alpha-2 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-PRKAA2 antibody
Anti-AMPK antibody
Anti-AMPK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance