• Western blot analysis of lysates from HeLa cells, using p-PRC1 (Phospho-Thr481) antibody.
  • Western blot analysis of 293T VEC lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Phospho-PRC1-Thr481 antibody (481 aa) (STJ98682)

SKU:
STJ98682

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein regulator of cytokinesis 1-Thr481 (481 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRC1
Gene ID: 9055
Uniprot ID: PRC1_HUMAN
Immunogen Region: 481 aa
Specificity: The antibody detects endogenous PRC1Phospho occurs at Thr481
Immunogen: Synthetic Phospho peptide from the human protein at the amino acid range 481
Post Translational Modifications Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. Phosphorylation by CDK1 prevents PLK1-binding: upon degradation of CDK1 at anaphase and dephosphorylation, it is then phosphorylated by PLK1, leading to cytokinesis.
Function Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
Protein Name Protein Regulator Of Cytokinesis 1
Database Links Reactome: R-HSA-5625900
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle Pole
Midbody
Chromosome
Colocalized With Kif20b In The Nucleus Of Bladder Carcinoma Cells At The Interphase
Colocalized With Kif20b In Bladder Carcinoma Cells At Prophase
Metaphase
Early Anaphase
At The Midzone In Late Anaphase And At The Contractile Ring In Telophase
Predominantly Localized To The Nucleus Of Interphase Cells
During Mitosis Becomes Associated With The Mitotic Spindle Poles And Localizes With The Cell Midbody During Cytokinesis
Co-Localizes With Prc1 In Early Mitosis And At The Spindle Midzone From Anaphase B To Telophase
Alternative Antibody Names Anti-Protein Regulator Of Cytokinesis 1 antibody
Anti-PRC1 antibody

Information sourced from Uniprot.org

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