• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PLD1-Thr147 antibody (STJ90647)

SKU:
STJ90647

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Phospholipase D1-Thr147 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLD1
Gene ID: 5337
Uniprot ID: PLD1_HUMAN
Specificity: Phospho-PC-PLD1 (T147) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at T147.
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human PC-PLD1 (phospho Thr147)
Function Function as phospholipase selective for phosphatidylcholine. Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
Protein Name Phospholipase D1
Pld 1
Hpld1
Choline Phosphatase 1
Phosphatidylcholine-Hydrolyzing Phospholipase D1
Database Links Reactome: R-HSA-1483148
Reactome: R-HSA-1483166
Reactome: R-HSA-2029485
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013408
Cellular Localisation Cytoplasm
Perinuclear Region
Endoplasmic Reticulum Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus Membrane
Late Endosome Membrane
Alternative Antibody Names Anti-Phospholipase D1 antibody
Anti-Pld 1 antibody
Anti-Hpld1 antibody
Anti-Choline Phosphatase 1 antibody
Anti-Phosphatidylcholine-Hydrolyzing Phospholipase D1 antibody
Anti-PLD1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance