• Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y1217 antibody (STJ117903) at 1:1000 dilution or PLCG2 antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of Phospho-PLC gamma 2 (PLCG2) -Y1217 in paraffin-embedded mouse spleen using Phospho-PLC gamma 2 (PLCG2) -Y1217 Rabbit polyclonal antibody (STJ117903) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-PLCG2-Y1217 antibody (STJ117903) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from Raji cells, using Phospho-PLC gamma 2 (PLCG2) -Y1217 Rabbit polyclonal antibody (STJ117903) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-PLCG2-Y1217 antibody (STJ117903)

SKU:
STJ117903

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PLCG2-Y1217 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCG2
Gene ID: 5336
Uniprot ID: PLCG2_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y1217 of human PLC gamma 2 (PLCG2) (NP_002652.2).
Immunogen Sequence: FLYDT
Post Translational Modifications Phosphorylated on tyrosine residues by CSF1R. Phosphorylated on tyrosine residues by BTK and SYK.upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity.
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-2
Phosphoinositide Phospholipase C-Gamma-2
Phospholipase C-Iv
Plc-Iv
Phospholipase C-Gamma-2
Plc-Gamma-2
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-166016
Reactome: R-HSA-1855204
Reactome: R-HSA-202433
Reactome: R-HSA-2029485
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Reactome: R-HSA-5607764
Reactome: R-HSA-5621480
Reactome: R-HSA-9027277
Reactome: R-HSA-9664323
Reactome: R-HSA-9679191
Reactome: R-HSA-9680350
Reactome: R-HSA-983695
Cellular Localisation Membrane Raft
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-2 antibody
Anti-Phosphoinositide Phospholipase C-Gamma-2 antibody
Anti-Phospholipase C-Iv antibody
Anti-Plc-Iv antibody
Anti-Phospholipase C-Gamma-2 antibody
Anti-Plc-Gamma-2 antibody
Anti-PLCG2 antibody

Information sourced from Uniprot.org

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