• Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using PEA-15 (Phospho-Ser116) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from COS7 cells treated with INSULIN 0.01U/ML 15', using PEA-15 (Phospho-Ser116) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of COLO 3T3 using p-PEA-15 (S116) antibody. Antibody was diluted at 1:500

Anti-Phospho-PEA15-Ser116 antibody (81-130 aa) (STJ90829)

SKU:
STJ90829

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Astrocytic phosphoprotein PEA-15-Ser116 (81-130 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEA15
Gene ID: 8682
Uniprot ID: PEA15_HUMAN
Immunogen Region: 81-130 aa
Specificity: Phospho-PEA-15 (S116) Polyclonal Antibody detects endogenous levels of PEA-15 protein only when phosphorylated at S116.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PEA-15 around the phosphorylation site of Ser116 at the amino acid range 81-130
Post Translational Modifications Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
Function Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Protein Name Astrocytic Phosphoprotein Pea-15
15 Kda Phosphoprotein Enriched In Astrocytes
Phosphoprotein Enriched In Diabetes
Ped
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-5673001
Cellular Localisation Cytoplasm
Associated With Microtubules
Alternative Antibody Names Anti-Astrocytic Phosphoprotein Pea-15 antibody
Anti-15 Kda Phosphoprotein Enriched In Astrocytes antibody
Anti-Phosphoprotein Enriched In Diabetes antibody
Anti-Ped antibody
Anti-PEA15 antibody

Information sourced from Uniprot.org

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