• Immunohistochemistry analysis of paraffin-embedded human brain, using PDCD4 (Phospho-Ser457) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from 293 cells treated with serum 20% 15', using PDCD4 (Phospho-Ser457) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-Pdcd-4 (S457) Polyclonal Antibody

Anti-Phospho-PDCD4-Ser457 antibody (420-469 aa) (STJ90921)

SKU:
STJ90921

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Programmed cell death protein 4-Ser457 (420-469 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDCD4
Gene ID: 27250
Uniprot ID: PDCD4_HUMAN
Immunogen Region: 420-469 aa
Specificity: Phospho-Pdcd-4 (S457) Polyclonal Antibody detects endogenous levels of Pdcd-4 protein only when phosphorylated at S457.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PDCD4 around the phosphorylation site of Ser457 at the amino acid range 420-469
Post Translational Modifications Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens.phosphorylation promotes proteasomal degradation of PDCD4.
Function Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Protein Name Programmed Cell Death Protein 4
Neoplastic Transformation Inhibitor Protein
Nuclear Antigen H731-Like
Protein 197/15a
Database Links Reactome: R-HSA-8950505
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And Cytoplasm
Predominantly Nuclear Under Normal Growth Conditions
And When Phosphorylated At Ser-457
Alternative Antibody Names Anti-Programmed Cell Death Protein 4 antibody
Anti-Neoplastic Transformation Inhibitor Protein antibody
Anti-Nuclear Antigen H731-Like antibody
Anti-Protein 197/15a antibody
Anti-PDCD4 antibody
Anti-H731 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance