• Western blot analysis of lysates from Jurkat cells treated with TNF-a 20ng/ml 30', using IkappaB-epsilon (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NFKBIE-Ser22 antibody (131-180 aa) (STJ91018)

SKU:
STJ91018

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NF-kappa-B inhibitor epsilon-Ser22 (131-180 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIE
Gene ID: 4794
Uniprot ID: IKBE_HUMAN
Immunogen Region: 131-180 aa
Specificity: Phospho-I Kappa B-Epsilon (S22) Polyclonal Antibody detects endogenous levels of I Kappa B-Epsilon protein only when phosphorylated at S22.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IkappaB-epsilon around the phosphorylation site of Ser22 at the amino acid range 131-180
Post Translational Modifications Serine phosphorylated.followed by proteasome-dependent degradation.
Function Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Protein Name Nf-Kappa-B Inhibitor Epsilon
Nf-Kappa-Bie
I-Kappa-B-Epsilon
Ikb-E
Ikb-Epsilon
Ikappabepsilon
Database Links Reactome: R-HSA-1169091
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Epsilon antibody
Anti-Nf-Kappa-Bie antibody
Anti-I-Kappa-B-Epsilon antibody
Anti-Ikb-E antibody
Anti-Ikb-Epsilon antibody
Anti-Ikappabepsilon antibody
Anti-NFKBIE antibody
Anti-IKBE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance