Anti-Phospho-NFKBIE-Ser22 antibody (131-180 aa) (STJ91018)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human IkappaB-epsilon around the phosphorylation site of Ser22 at the amino acid range 131-180
General Information
| Short Description | Rabbit polyclonal anti-Phospho-NF-kappa-B inhibitor epsilon-Ser22 (131-180 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NFKBIE |
| Gene ID | 4794 |
| Uniprot ID | IKBE_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human IkappaB-epsilon around the phosphorylation site of Ser22 at the amino acid range 131-180 |
| Immunogen Region | 131-180 aa |
| Specificity | Phospho-I Kappa B-Epsilon (S22) Polyclonal Antibody detects endogenous levels of I Kappa B-Epsilon protein only when phosphorylated at S22. |
Additional Info
| Post Translational Modifications | Serine phosphorylated.followed by proteasome-dependent degradation. |
| Function | Sequesters NF-kappa-B transcription factor complexes in the cytoplasm, thereby inhibiting their activity. Sequestered complexes include NFKB1-RELA (p50-p65) and NFKB1-REL (p50-c-Rel) complexes. Limits B-cell activation in response to pathogens, and also plays an important role in B-cell development. |
| Protein Name | Nf-Kappa-B Inhibitor EpsilonNf-Kappa-BieI-Kappa-B-EpsilonIkb-EIkb-EpsilonIkappabepsilon |
| Database Links | Reactome: R-HSA-1169091 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Nf-Kappa-B Inhibitor Epsilon antibodyAnti-Nf-Kappa-Bie antibodyAnti-I-Kappa-B-Epsilon antibodyAnti-Ikb-E antibodyAnti-Ikb-Epsilon antibodyAnti-Ikappabepsilon antibodyAnti-NFKBIE antibodyAnti-IKBE antibody |
Information sourced from Uniprot.org