| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Nuclear factor of activated T-cells 5-Ser1197 (1171-1220 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFAT5 |
| Gene ID: |
10725 |
| Uniprot ID: |
NFAT5_HUMAN |
| Immunogen Region: |
1171-1220 aa |
| Specificity: |
Phospho-NFAT5 (S1197) Polyclonal Antibody detects endogenous levels of NFAT5 protein only when phosphorylated at S1197. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human NFAT5 around the phosphorylation site of Ser1197 at the amino acid range 1171-1220 |
| Post Translational Modifications | Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress.this phosphorylation mediates its rapid nuclear localization. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage. |
| Function | Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-ACTAGTGGAAACATATAATCCAATGGTGACCGCT-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes.optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. |
| Protein Name | Nuclear Factor Of Activated T-Cells 5Nf-At5T-Cell Transcription Factor Nfat5Tonicity-Responsive Enhancer-Binding ProteinTone-Binding ProteinTonebp |
| Cellular Localisation | NucleusCytoplasmChromosomeNuclear Distribution Increases Under Hypertonic ConditionsRecruited To Sites Of R-Loop-Associated Dna Damage Following Poly-Adp-Ribosylation By Parp1 |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells 5 antibodyAnti-Nf-At5 antibodyAnti-T-Cell Transcription Factor Nfat5 antibodyAnti-Tonicity-Responsive Enhancer-Binding Protein antibodyAnti-Tone-Binding Protein antibodyAnti-Tonebp antibodyAnti-NFAT5 antibodyAnti-KIAA0827 antibodyAnti-TONEBP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
