• Western blot analysis of extracts of C6 cells, using Phospho-NDRG1-T346 antibody (STJ117890) at 1:1000 dilution. C6 cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from C6 cells, using Phospho-NDRG1-T346 Rabbit polyclonal antibody (STJ117890) at 1:1000 dilution. C6 cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-NDRG1-T346 antibody (STJ117890)

SKU:
STJ117890

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NDRG1-T346 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDRG1
Gene ID: 10397
Uniprot ID: NDRG1_HUMAN
Immunogen: A synthetic phosphorylated peptide around T346 of human NDRG1 (NP_001128714.1).
Immunogen Sequence: SHTSE
Tissue Specificity Ubiquitous.expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
Post Translational Modifications Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent.
Function Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
Protein Name Protein Ndrg1
Differentiation-Related Gene 1 Protein
Drg-1
N-Myc Downstream-Regulated Gene 1 Protein
Nickel-Specific Induction Protein Cap43
Reducing Agents And Tunicamycin-Responsive Protein
Rtp
Rit42
Database Links Reactome: R-HSA-6803205
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Cell Membrane
Mainly Cytoplasmic But Differentially Localized To Other Regions
Associates With The Plasma Membrane In Intestinal Epithelia And Lactating Mammary Gland
Translocated To The Nucleus In A P53/Tp53-Dependent Manner
In Prostate Epithelium And Placental Chorion
Located In Both The Cytoplasm And In The Nucleus
No Nuclear Localization In Colon Epithelium Cells
In Intestinal Mucosa
Prostate And Renal Cortex
Located Predominantly Adjacent To Adherens Junctions
Cytoplasmic With Granular Staining In Proximal Tubular Cells Of The Kidney And Salivary Gland Ducts
Recruits To The Membrane Of Recycling/Sorting And Late Endosomes Via Binding To Phosphatidylinositol 4-Phosphate
Associates With Microtubules
Colocalizes With Tubg1 In The Centrosome
Cytoplasmic Location Increased With Hypoxia
Phosphorylated Form Found Associated With Centromeres During S-Phase Of Mitosis And With The Plasma Membrane
Alternative Antibody Names Anti-Protein Ndrg1 antibody
Anti-Differentiation-Related Gene 1 Protein antibody
Anti-Drg-1 antibody
Anti-N-Myc Downstream-Regulated Gene 1 Protein antibody
Anti-Nickel-Specific Induction Protein Cap43 antibody
Anti-Reducing Agents And Tunicamycin-Responsive Protein antibody
Anti-Rtp antibody
Anti-Rit42 antibody
Anti-NDRG1 antibody
Anti-CAP43 antibody
Anti-DRG1 antibody
Anti-RTP antibody

Information sourced from Uniprot.org

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