• Western blot analysis of extracts from 293 cells, using Phospho-MARCKS-S162 antibody (STJ22280). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of various lysates, using Phospho-MARCKS-S162 antibody (STJ22280) at 1:400 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-MARCKS-S162 antibody (STJ22280).

Anti-Phospho-MARCKS-S162 antibody (STJ22280)

SKU:
STJ22280

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARCKS
Gene ID: 4082
Uniprot ID: MARCS_HUMAN
Specificity: A phospho specific peptide corresponding to residues surrounding S162 of human MARCKS
Tissue Specificity Detected in spermatozoa.
Post Translational Modifications Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. PKC-mediated phosphorylation increases 4 to 5-fold upon TNF-alpha or LPS induction. Myristoylated. A proper myristoylation is essential for the proper distribution to the plasma membrane. Acetylated at Lys-172 by KAT5.acetylation is required for its subsequent phosphorylation. Deacetylated by SIRT2.
Function Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes. Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages. Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type. Participates in the regulation of neurite initiation and outgrowth by interacting with components of cellular machinery including CDC42 that regulates cell shape and process extension through modulation of the cytoskeleton. Plays also a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane.
Protein Name Myristoylated Alanine-Rich C-Kinase Substrate
Marcks
Protein Kinase C Substrate - 80 Kda Protein - Light Chain
80k-L Protein
Pkcsl
Database Links Reactome: R-HSA-399997
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasm
Cytoskeleton
Pkc-Dependent Phosphorylation Displaces Marcks From The Cell Membrane And Subsequent Dephosphorylation Is Accompanied By Its Reassociation With The Membrane
Alternative Antibody Names Anti-Myristoylated Alanine-Rich C-Kinase Substrate antibody
Anti-Marcks antibody
Anti-Protein Kinase C Substrate - 80 Kda Protein - Light Chain antibody
Anti-80k-L Protein antibody
Anti-Pkcsl antibody
Anti-MARCKS antibody
Anti-MACS antibody
Anti-PRKCSL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance