• Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-MAP2K5-Ser311/Thr315 antibody (STJA0003686)

SKU:
STJA0003686-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Xenopus/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MEK5-Ser311/Thr315 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Map2k5
Gene ID: 29568
Uniprot ID: MP2K5_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH.
Tissue Specificity MEK5 beta is ubiquitously distributed with highest levels in the liver, whereas MEK5 alpha is expressed only in liver and brain.
Post Translational Modifications Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Function Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 5
Map Kinase Kinase 5
Mapkk 5
Mapk/Erk Kinase 5
Mek 5
Cellular Localisation Isoform Beta: Cytoplasm
Cytosol
Isoform Alpha-1: Membrane
The Alternatively Spliced Exon In Alpha Isoform Resemble Conserved Sequences That Mediate Interactions With The Cytoskeleton
Thereby Explaining The Differential Localization
Isoform Alpha-2: Membrane
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 5 antibody
Anti-Map Kinase Kinase 5 antibody
Anti-Mapkk 5 antibody
Anti-Mapk/Erk Kinase 5 antibody
Anti-Mek 5 antibody
Anti-Map2k5 antibody
Anti-Mek5 antibody
Anti-Mkk5 antibody
Anti-Prkmk5 antibody

Information sourced from Uniprot.org

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