Host: | Rabbit |
Applications: | WB/IF/ICC |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:2000 IF/ICC:1:100-1:200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | MAP2K2 |
Gene ID: | 5605 |
Uniprot ID: | MP2K2_HUMAN |
Specificity: | A phospho specific peptide corresponding to residues surrounding T394 of human MEK2 |
Post Translational Modifications | MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1. (Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. |
Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 Map Kinase Kinase 2 Mapkk 2 Erk Activator Kinase 2 Mapk/Erk Kinase 2 Mek 2 |
Database Links | Reactome: R-HSA-112411 Reactome: R-HSA-170968 Reactome: R-HSA-445144 Reactome: R-HSA-5210891 Reactome: R-HSA-5673000 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9652169 Reactome: R-HSA-9656223 |
Cellular Localisation | Cytoplasm Membrane Peripheral Membrane Protein Membrane Localization Is Probably Regulated By Its Interaction With Ksr1 |
Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibody Anti-Map Kinase Kinase 2 antibody Anti-Mapkk 2 antibody Anti-Erk Activator Kinase 2 antibody Anti-Mapk/Erk Kinase 2 antibody Anti-Mek 2 antibody Anti-MAP2K2 antibody Anti-MEK2 antibody Anti-MKK2 antibody Anti-PRKMK2 antibody |
Information sourced from Uniprot.org