| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Mitotic spindle assembly checkpoint protein MAD1-Ser428 (394-443 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAD1L1 |
| Gene ID: |
8379 |
| Uniprot ID: |
MD1L1_HUMAN |
| Immunogen Region: |
394-443 aa |
| Specificity: |
Phospho-MAD1 (S428) Polyclonal Antibody detects endogenous levels of MAD1 protein only when phosphorylated at S428. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human MAD1 around the phosphorylation site of Ser428 at the amino acid range 394-443 |
| Post Translational Modifications | Phosphorylated.by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. Phosphorylated.by TTK. |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling. Isoform 3: Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas. |
| Protein Name | Mitotic Spindle Assembly Checkpoint Protein Mad1Mitotic Arrest Deficient 1-Like Protein 1Mad1-Like Protein 1Mitotic Checkpoint Mad1 Protein HomologHsmad1Hmad1Tax-Binding Protein 181 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreNucleus EnvelopeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleSpindle PoleCo-Localizes With Tpr At The Nucleus Envelope During Interphase And Throughout The Cell CycleFrom The Beginning To The End Of MitosisIt Is Seen To Move From A Diffusely Nuclear Distribution To The CentrosomeTo The Spindle Midzone And Finally To The MidbodyLocalizes To Kinetochores During PrometaphaseDoes Not Localize To Kinetochores During MetaphaseColocalizes With Nek2 At The KinetochoreColocalizes With Ik At Spindle Poles During Metaphase And AnaphaseIsoform 3: Cytoplasm |
| Alternative Antibody Names | Anti-Mitotic Spindle Assembly Checkpoint Protein Mad1 antibodyAnti-Mitotic Arrest Deficient 1-Like Protein 1 antibodyAnti-Mad1-Like Protein 1 antibodyAnti-Mitotic Checkpoint Mad1 Protein Homolog antibodyAnti-Hsmad1 antibodyAnti-Hmad1 antibodyAnti-Tax-Binding Protein 181 antibodyAnti-MAD1L1 antibodyAnti-MAD1 antibodyAnti-TXBP181 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
