• Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 8 (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1：500
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1：500

Anti-Phospho-KRT8-Ser73 antibody (41-90 aa) (STJ90242)

SKU:
STJ90242

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Keratin, type II cytoskeletal 8-Ser73 (41-90 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT8
Gene ID: 3856
Uniprot ID: K2C8_HUMAN
Immunogen Region: 41-90 aa
Specificity: Phospho-Cytokeratin 8 (S73) Polyclonal Antibody detects endogenous levels of Cytokeratin 8 protein only when phosphorylated at S73.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Keratin 8 around the phosphorylation site of Ser73 at the amino acid range 41-90
Post Translational Modifications Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Name Keratin - Type Ii Cytoskeletal 8
Cytokeratin-8
Ck-8
Keratin-8
K8
Type-Ii Keratin Kb8
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleus Matrix
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 8 antibody
Anti-Cytokeratin-8 antibody
Anti-Ck-8 antibody
Anti-Keratin-8 antibody
Anti-K8 antibody
Anti-Type-Ii Keratin Kb8 antibody
Anti-KRT8 antibody
Anti-CYK8 antibody

Information sourced from Uniprot.org

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