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Anti-Phospho-KRAS-S89 antibody (STJ11102656)

Western blot analysis of extracts of various cell lines, using Phospho-KRAS-S89 rabbit polyclonal antibody (STJ11102656) at 1:500 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry analysis of Phospho-KRAS-S89 in paraffin-embedded mouse kidney using Phospho-KRAS-S89 Rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using Phospho-KRAS-S89 rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of Phospho-KRAS-S89 in paraffin-embedded rat brain using Phospho-KRAS-S89 Rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-KRAS-S89 rabbit polyclonal antibody (STJ11102656) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Western blot analysis of various lysates using Phospho-KRAS-S89 Rabbit polyclonal antibody (STJ11102656) at 1:500 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-KRAS-S89 antibody (STJ11102656)

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STJ11102656

£146.50 - £639.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-KRAS-S89 is suitable for use in Western Blot and Immunohistochemistry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	KRAS
Gene ID:	3845
Uniprot ID:	RASK_HUMAN
Immunogen:	A synthetic phosphorylated peptide around S89 of human KRAS (NP_001356715.1).
Immunogen Sequence:	TKSFE

Post Translational Modifications	Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180. Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.
Protein Name	Gtpase Kras K-Ras 2 Ki-Ras C-K-Ras C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed
Database Links	Reactome: R-HSA-112412 Reactome: R-HSA-1169092 Reactome: R-HSA-1236382 Reactome: R-HSA-1250196 Reactome: R-HSA-1250347 Reactome: R-HSA-1433557 Reactome: R-HSA-167044 Reactome: R-HSA-171007 Reactome: R-HSA-179812 Reactome: R-HSA-180336 Reactome: R-HSA-186763 Reactome: R-HSA-1963640 Reactome: R-HSA-210993 Reactome: R-HSA-2179392 Reactome: R-HSA-2424491 Reactome: R-HSA-2428933 Reactome: R-HSA-2871796 Reactome: R-HSA-375165 Reactome: R-HSA-4086398 P01116-2 Reactome: R-HSA-442982 Reactome: R-HSA-5218921 Reactome: R-HSA-5621575 Reactome: R-HSA-5637810 Reactome: R-HSA-5654688 Reactome: R-HSA-5654693 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658442 Reactome: R-HSA-5673000 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802953 Reactome: R-HSA-6802955 Reactome: R-HSA-74751 Reactome: R-HSA-8849471 Reactome: R-HSA-8851805 Reactome: R-HSA-8951936 Reactome: R-HSA-9026519 Reactome: R-HSA-9027284 Reactome: R-HSA-9028731 Reactome: R-HSA-9034864 Reactome: R-HSA-9607240 Reactome: R-HSA-9634285 Reactome: R-HSA-9634635 Reactome: R-HSA-9648002 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674555 Reactome: R-HSA-9680350 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9753510 Reactome: R-HSA-9753512
Cellular Localisation	Cell Membrane Lipid-Anchor Cytoplasmic Side Endomembrane System Cytoplasm Cytosol Isoform 2b: Cell Membrane
Alternative Antibody Names	Anti-Gtpase Kras antibody Anti-K-Ras 2 antibody Anti-Ki-Ras antibody Anti-C-K-Ras antibody Anti-C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed antibody Anti-KRAS antibody Anti-KRAS2 antibody Anti-RASK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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