• Immunohistochemistry analysis of paraffin-embedded human brain, using Kv2.1/KCNB1 (Phospho-Ser567) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HepG2 cells, using Kv2.1/KCNB1 (Phospho-Ser567) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-KCNB1-Ser567 antibody (533-582 aa) (STJ91316)

SKU:
STJ91316

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Potassium voltage-gated channel subfamily B member 1-Ser567 (533-582 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNB1
Gene ID: 3745
Uniprot ID: KCNB1_HUMAN
Immunogen Region: 533-582 aa
Specificity: Phospho-Kv2.1 (S567) Polyclonal Antibody detects endogenous levels of Kv2.1 protein only when phosphorylated at S567.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Kv2.1/KCNB1 around the phosphorylation site of Ser567 at the amino acid range 533-582
Post Translational Modifications Phosphorylated. Differential C-terminal phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating in hippocampal neurons. Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/dephosphorylation activities. Tyr-128 can be phosphorylated by Src and dephosphorylated by cytoplasmic form of the phosphatase PTPRE. CDK5-induced Ser-607 phosphorylation increases in response to acute blockade of neuronal activity. Phosphorylated on Tyr-128 by Src and on Ser-805 by MAPK14/P38MAPK.phosphorylations are necessary and sufficient for an increase in plasma membrane insertion, apoptotic potassium current surge and completion of the neuronal cell death program. Phosphorylated on Ser-520, Ser-607, Ser-656 and Ser-805 by CDK5.phosphorylation is necessary for KCNB1 channel clustering formation. The Ser-607 phosphorylation state differs between KCNB1-containing clusters on the proximal and distal portions of the axon initial segment (AIS). Highly phosphorylated on serine residues in the C-terminal cytoplasmic tail in resting neurons. Phosphorylated in pancreatic beta cells in response to incretin hormones stimulation in a PKA- and RPS6KA5/MSK1-dependent signaling pathway, promoting beta cell survival. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5.levels then increase to reach adult levels by P14. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are sensitive to calcineurin-mediated dephosphorylation contributing to the modulation of the voltage-dependent gating properties. Dephosphorylation by phosphatase PTPRE confers neuroprotection by its inhibitory influence on the neuronal apoptotic potassium current surge in a Zn(2+)-dependent manner. Dephosphorylated at Ser-607 by protein phosphatase PPP1CA. Hypoxia-, seizure- or glutamate-induced neuronal activity promote calcium/calcineurin-dependent dephosphorylation resulting in a loss of KCNB1-containing clustering and enhanced channel activity. In response to brain ischemia, Ser-567 and Ser-607 are strongly dephosphorylated while Ser-457 and Ser-720 are less dephosphorylated. In response to brain seizures, phosphorylation levels on Ser-567 and Ser-607 are greatly reduced. Phosphorylated/dephosphorylated by Src or FYN tyrosine-protein kinases and tyrosine phosphatase PTPRE in primary Schwann cells and sciatic nerve tissue. Phosphorylation at Ser-593 of the FFAT motif activates interaction with MOSPD2, VAPA and VAPB. Acetylated. Acetylation occurs in pancreatic beta cells in response to stimulation by incretin hormones in a histone acetyltransferase (HAT)/histone deacetylase (HDAC)-dependent signaling pathway, promoting beta cell survival. Sumoylated on Lys-474, preferentially with SUMO1.sumoylation induces a positive shift in the voltage-dependence of activation and inhibits channel activity. Sumoylation increases the frequency of repetitive action potential firing at the cell surface of hippocampal neurons and decreases its frequency in pancreatic beta cells. Desumoylated by SENP1.
Function Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain, but also in the pancreas and cardiovascular system. Contributes to the regulation of the action potential (AP) repolarization, duration and frequency of repetitive AP firing in neurons, muscle cells and endocrine cells and plays a role in homeostatic attenuation of electrical excitability throughout the brain. Plays also a role in the regulation of exocytosis independently of its electrical function. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization. Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB2.channel properties depend on the type of alpha subunits that are part of the channel. Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNF1, KCNG1, KCNG3, KCNG4, KCNH1, KCNH2, KCNS1, KCNS2, KCNS3 and KCNV1, creating a functionally diverse range of channel complexes. Heterotetrameric channel activity formed with KCNS3 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells. Channel properties are also modulated by cytoplasmic ancillary beta subunits such as AMIGO1, KCNE1, KCNE2 and KCNE3, slowing activation and inactivation rate of the delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Major contributor to the slowly inactivating delayed-rectifier voltage-gated potassium current in neurons of the central nervous system, sympathetic ganglion neurons, neuroendocrine cells, pancreatic beta cells, cardiomyocytes and smooth muscle cells. Mediates the major part of the somatodendritic delayed-rectifier potassium current in hippocampal and cortical pyramidal neurons and sympathetic superior cervical ganglion (CGC) neurons that acts to slow down periods of firing, especially during high frequency stimulation. Plays a role in the induction of long-term potentiation (LTP) of neuron excitability in the CA3 layer of the hippocampus. Contributes to the regulation of glucose-induced action potential amplitude and duration in pancreatic beta cells, hence limiting calcium influx and insulin secretion. Plays a role in the regulation of resting membrane potential and contraction in hypoxia-treated pulmonary artery smooth muscle cells. May contribute to the regulation of the duration of both the action potential of cardiomyocytes and the heart ventricular repolarization QT interval. Contributes to the pronounced pro-apoptotic potassium current surge during neuronal apoptotic cell death in response to oxidative injury. May confer neuroprotection in response to hypoxia/ischemic insults by suppressing pyramidal neurons hyperexcitability in hippocampal and cortical regions. Promotes trafficking of KCNG3, KCNH1 and KCNH2 to the cell surface membrane, presumably by forming heterotetrameric channels with these subunits. Plays a role in the calcium-dependent recruitment and release of fusion-competent vesicles from the soma of neurons, neuroendocrine and glucose-induced pancreatic beta cells by binding key components of the fusion machinery in a pore-independent manner.
Protein Name Potassium Voltage-Gated Channel Subfamily B Member 1
Delayed Rectifier Potassium Channel 1
Drk1
H-Drk1
Voltage-Gated Potassium Channel Subunit Kv2.1
Database Links Reactome: R-HSA-1296072
Reactome: R-HSA-381676
Cellular Localisation Cell Membrane
Perikaryon
Cell Projection
Axon
Dendrite
Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Synapse
Synaptosome
Lateral Cell Membrane
Sarcolemma
Localizes To High-Density Somatodendritic Clusters And Non-Clustered Sites On The Surface Of Neocortical And Hippocampal Pyramidal Neurons In A Cortical Actin Cytoskeleton-Dependent Manner
Localizes Also To High-Density Clusters In The Axon Initial Segment (Ais)
At Ankyrin-G-Deficient Sites
On The Surface Of Neocortical And Hippocampal Pyramidal Neurons
Kcnb1-Containing Ais Clusters Localize Either In Close Apposition To Smooth Endoplasmic Reticulum Cisternal Organelles Or With Gaba-A Receptor-Containing Synapses Of Hippocampal And Cortical Pyramidal Neurons
Respectively
Localizes To High-Density Clusters On The Cell Surface Of Atrial And Ventricular Myocytes And At The Lateral Plasma Membrane In Epithelial Cells
Localizes Both To The Axial And Transverse Tubules (T Tubule) And Sarcolemma In Ventricular Myocytes
Associated With Lipid Raft Domains
In Cortical Neurons
Apoptotic Injuries Induce De Novo Plasma Membrane Insertion In A Snare-Dependent Manner Causing An Apoptotic Potassium Current Surge
Alternative Antibody Names Anti-Potassium Voltage-Gated Channel Subfamily B Member 1 antibody
Anti-Delayed Rectifier Potassium Channel 1 antibody
Anti-Drk1 antibody
Anti-H-Drk1 antibody
Anti-Voltage-Gated Potassium Channel Subunit Kv2.1 antibody
Anti-KCNB1 antibody

Information sourced from Uniprot.org

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