• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-HTT-Ser421 antibody (387-436 aa) (STJ91160)

SKU:
STJ91160

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Huntingtin-Ser421 (387-436 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTT
Gene ID: 3064
Uniprot ID: HD_HUMAN
Immunogen Region: 387-436 aa
Specificity: Phospho-Huntingtin (S421) Polyclonal Antibody detects endogenous levels of Huntingtin protein only when phosphorylated at S421.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Huntingtin around the phosphorylation site of Ser421 at the amino acid range 387-436
Post Translational Modifications Huntingtin: Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis. Huntingtin: Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. Huntingtin: Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Huntingtin, myristoylated N-terminal fragment: Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.
Function Huntingtin: May play a role in microtubule-mediated transport or vesicle function. Huntingtin, myristoylated N-terminal fragment: Promotes the formation of autophagic vesicles.
Protein Name Huntingtin
Huntington Disease Protein
Hd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment
Database Links Reactome: R-HSA-9022692
Cellular Localisation Huntingtin: Cytoplasm
Nucleus
Early Endosome
The Mutant Huntingtin Protein Colocalizes With Akap8l In The Nuclear Matrix Of Huntington Disease Neurons
Shuttles Between Cytoplasm And Nucleus In A Ran Gtpase-Independent Manner
Recruits Onto Early Endosomes In A Rab5- And Hap40-Dependent Fashion
Huntingtin
Myristoylated N-Terminal Fragment: Cytoplasmic Vesicle
Autophagosome
Alternative Antibody Names Anti-Huntingtin antibody
Anti-Huntington Disease Protein antibody
Anti-Hd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment antibody
Anti-HTT antibody
Anti-HD antibody
Anti-IT15 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance