Anti-Phospho-GYS1-S641 antibody (STJ11101015)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101015
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General Information

Short DescriptionRabbit polyclonal Phospho-GYS1-S641 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGYS1
Gene ID2997
Uniprot IDGYS1_HUMAN
Immunogen SequencePASVP
SpecificityA synthetic phosphorylated peptide around S641 of human Glycogen synthase 1 (GYS1) (NP_002094.2).

Additional Info

Tissue Specificity Expressed in skeletal muscle and most other cell types where glycogen is present.
Post Translational Modifications Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Phosphorylated at Ser-641 by DYRK2, leading to inactivation. Phosphorylated at Ser-641 by PASK, leading to inactivation.phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Function Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Protein Name Glycogen Starch Synthase - Muscle
Glycogen Synthase 1
Database Links Reactome: R-HSA-3322077
Reactome: R-HSA-3785653
Reactome: R-HSA-3814836
Reactome: R-HSA-3828062
Cellular Localisation
Alternative Antibody Names Anti-Glycogen Starch Synthase - Muscle antibody
Anti-Glycogen Synthase 1 antibody
Anti-GYS1 antibody
Anti-GYS antibody

Information sourced from Uniprot.org

Citations

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