Anti-Phospho-GTF2I-Tyr248 antibody (214-263 aa) (STJ90480)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TFII-I around the phosphorylation site of Tyr248 at the amino acid range 214-263
General Information
| Short Description | Rabbit polyclonal anti-Phospho-General transcription factor II-I-Tyr248 (214-263 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GTF2I |
| Gene ID | 2969 |
| Uniprot ID | GTF2I_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TFII-I around the phosphorylation site of Tyr248 at the amino acid range 214-263 |
| Immunogen Region | 214-263 aa |
| Specificity | Phospho-TFII-I (Y248) Polyclonal Antibody detects endogenous levels of TFII-I protein only when phosphorylated at Y248. |
Additional Info
| Post Translational Modifications | Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation. Sumoylated. |
| Function | Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. |
| Protein Name | General Transcription Factor Ii-IGtfii-ITfii-IBruton Tyrosine Kinase-Associated Protein 135Bap-135Btk-Associated Protein 135Srf-Phox1-Interacting ProteinSpinWilliams-Beuren Syndrome Chromosomal Region 6 Protein |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusColocalizes With Btk In The Cytoplasm |
| Alternative Antibody Names | Anti-General Transcription Factor Ii-I antibodyAnti-Gtfii-I antibodyAnti-Tfii-I antibodyAnti-Bruton Tyrosine Kinase-Associated Protein 135 antibodyAnti-Bap-135 antibodyAnti-Btk-Associated Protein 135 antibodyAnti-Srf-Phox1-Interacting Protein antibodyAnti-Spin antibodyAnti-Williams-Beuren Syndrome Chromosomal Region 6 Protein antibodyAnti-GTF2I antibodyAnti-BAP135 antibodyAnti-WBSCR6 antibody |
Information sourced from Uniprot.org