• Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1336 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).
  • Immunostaining of mouse dentate gyrus 48 hours post TMT treatment showing NR2B when phosphorylated at Tyr1336 (green, 1:400). The blue is staining nuclei using DAPI. Photo Courtesy of Rob Wine.

Anti-Phospho-GRIN2B-Tyr1336 antibody (STJA0003721)

SKU:
STJA0003721-100

£442.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat/Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NMDAR2B-Tyr1336 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC 1:400
ICC 1:100
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Grin2b
Gene ID: 24410
Uniprot ID: NMDE2_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 conjugated to KLH.
Tissue Specificity Expressed in the hippocampus including the dentate gyrus (at protein level). Detected in adult olfactory bulb, brain cortex, hippocampus, striatum, thalamus, superior colliculus, with much lower levels in inferior colliculus, midbrain and cerebellum.
Post Translational Modifications Phosphorylated on tyrosine residues. Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity.
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity.
Protein Name Glutamate Receptor Ionotropic - Nmda 2b
Glun2b
Glutamate Nmda Receptor Subunit Epsilon-2
N-Methyl D-Aspartate Receptor Subtype 2b
Nmdar2b
Nr2b
Database Links Reactome: R-RNO-3928662
Reactome: -RNO-438066
Reactome: -RNO-5673001
Reactome: -RNO-8849932
Reactome: -RNO-9609736
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Late Endosome
Lysosome
Cytoplasm
Cytoskeleton
Co-Localizes With The Motor Protein Kif17 Along Microtubules
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Nmda 2b antibody
Anti-Glun2b antibody
Anti-Glutamate Nmda Receptor Subunit Epsilon-2 antibody
Anti-N-Methyl D-Aspartate Receptor Subtype 2b antibody
Anti-Nmdar2b antibody
Anti-Nr2b antibody
Anti-Grin2b antibody

Information sourced from Uniprot.org

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