Anti-Phospho-GATA1-Ser142 antibody (109-158 aa) (STJ91112)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GATA1 around the phosphorylation site of Ser142 at the amino acid range 109-158
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Erythroid transcription factor-Ser142 (109-158 aa) for use in WB, IHC, IF, IP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IP 2-5 ug mg/lysateELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GATA1 |
| Gene ID | 2623 |
| Uniprot ID | GATA1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GATA1 around the phosphorylation site of Ser142 at the amino acid range 109-158 |
| Immunogen Region | 109-158 aa |
| Specificity | Phospho-GATA-1 (S142) Polyclonal Antibody detects endogenous levels of GATA-1 protein only when phosphorylated at S142. |
Additional Info
| Post Translational Modifications | Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300. |
| Function | Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS. |
| Protein Name | Erythroid Transcription FactorEryf1Gata-Binding Factor 1Gata-1Gf-1Nf-E1 Dna-Binding Protein |
| Database Links | Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Erythroid Transcription Factor antibodyAnti-Eryf1 antibodyAnti-Gata-Binding Factor 1 antibodyAnti-Gata-1 antibodyAnti-Gf-1 antibodyAnti-Nf-E1 Dna-Binding Protein antibodyAnti-GATA1 antibodyAnti-ERYF1 antibodyAnti-GF1 antibody |
Information sourced from Uniprot.org